CTLA4_site_10 | SCGE Toolkit

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SCGE ID	10000000135
Lab ID	CTLA4_site_10
Source	lab IVT
Target Locus	CTLA4
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GAGGTTCACTTGATTTCCAC
Target Sequence + PAM	GAGGTTCACTTGATTTCCACNGG
Position	hg38/chr2:203870795-203870818

Spacer Sequence	GAGGUUCACUUGAUUUCCAC
Spacer Length	20
Modifications	none

Forward Primer	GCAAAGCCACTGAGGTCCGGGTG
Reverse Primer	GGGTTCCGTTGCCTATGCCCAGG

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	64
Guide-seq	1

Specificity Ratio	0.246

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: CTLA4_site_10 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)