CXCR4_site_03 | SCGE Toolkit

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SCGE ID	10000000141
Lab ID	CXCR4_site_03
Source	lab IVT
Target Locus	CXCR4
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GAAGATGATGGAGTAGATGG
Target Sequence + PAM	GAAGATGATGGAGTAGATGGNGG
Position	hg38/chr2:136115780-136115803

Spacer Sequence	GAAGAUGAUGGAGUAGAUGG
Spacer Length	20
Modifications	none

Forward Primer	CCCAAAGTACCAGTTTGCCACGGC
Reverse Primer	GGCTCAGGGGACTATGACTCCATGA

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	3321
Guide-seq	122

Specificity Ratio	0.004

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: CXCR4_site_03 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)