FAS_site_01 | SCGE Toolkit

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SCGE ID	10000000149
Lab ID	FAS_site_01
Source	lab IVT
Target Locus	FAS
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GAGGGCTCACCAGAGGTAGG
Target Sequence + PAM	GAGGGCTCACCAGAGGTAGGNGG
Position	hg38/chr10:88990893-88990916

Spacer Sequence	GAGGGCUCACCAGAGGUAGG
Spacer Length	20
Modifications	none

Forward Primer	CTCTTCTCCCGCGGGTTGGTGGA
Reverse Primer	GAGCCAGTCTCCAACGCCTCCCG

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	1920

Specificity Ratio	0.022

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: FAS_site_01 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)