FAS_site_02 | SCGE Toolkit

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SCGE ID	10000000150
Lab ID	FAS_site_02
Source	lab IVT
Target Locus	FAS
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GCTGACCCCGCTGGGCAGGC
Target Sequence + PAM	GCTGACCCCGCTGGGCAGGCNGG
Position	hg38/chr10:88991146-88991169

Spacer Sequence	GCUGACCCCGCUGGGCAGGC
Spacer Length	20
Modifications	none

Forward Primer	CGGGGGCTGTTAGGACCTTCCCT
Reverse Primer	AGGGCTTGTCCAGGAGTTCCGCT

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	867
Guide-seq	5

Specificity Ratio	0.176

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: FAS_site_02 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)