Development System
LAG3_site_03 | SCGE Toolkit
Targets endogenous human locus
10000000156 |
LAG3_site_03 |
lab IVT |
LAG3 |
human |
Targets endogenous human locus |
SpyCas9 |
|
GCTGCAGAAACAGCAAGCCC |
GCTGCAGAAACAGCAAGCCCNGG |
hg38/chr12:6772869-6772892 |
|
GCUGCAGAAACAGCAAGCCC |
20 |
none |
|
CCAAGGCCCTCTCCTGGTCTCCC |
GAGATCCCTGCTGGTCCCCTCCT |
Ivt Construct Source | Addgene |
Vector Id | 153997 |
Vector Name | pCRL01 |
Vector Description | Plasmid for single guide RNA IVT |
Vector Type | plasmid |
Annotated Map | addgene-plasmid-153997-sequence-304516 |
|
|
Detection Method | No. of sites detected |
Change-seq |
1136
|
Guide-seq |
5
|
|
|
Publication Title |
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI |
This Guide: LAG3_site_03 is being used ...
Project |
Initiative |
Contact PI |
|
|
Biological Effects |
Shengdar Q Tsai, PhD
(St. Jude Children's Research Hospital) |
|