LAG3_site_03 | SCGE Toolkit

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SCGE ID	10000000156
Lab ID	LAG3_site_03
Source	lab IVT
Target Locus	LAG3
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GCTGCAGAAACAGCAAGCCC
Target Sequence + PAM	GCTGCAGAAACAGCAAGCCCNGG
Position	hg38/chr12:6772869-6772892

Spacer Sequence	GCUGCAGAAACAGCAAGCCC
Spacer Length	20
Modifications	none

Forward Primer	CCAAGGCCCTCTCCTGGTCTCCC
Reverse Primer	GAGATCCCTGCTGGTCCCCTCCT

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	1136
Guide-seq	5

Specificity Ratio	0.153

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: LAG3_site_03 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)