SCGE ID10000000156
Lab IDLAG3_site_03
Sourcelab IVT
Target LocusLAG3
Specieshuman
DescriptionTargets endogenous human locus
Guide CompatibilitySpyCas9

Target SequenceGCTGCAGAAACAGCAAGCCC
Target Sequence + PAMGCTGCAGAAACAGCAAGCCCNGG
Positionhg38/chr12:6772869-6772892

Spacer SequenceGCUGCAGAAACAGCAAGCCC
Spacer Length20
Modificationsnone

Forward PrimerCCAAGGCCCTCTCCTGGTCTCCC
Reverse PrimerGAGATCCCTGCTGGTCCCCTCCT


  • SpCas9


  • Ivt Construct SourceAddgene
    Vector Id153997
    Vector NamepCRL01
    Vector DescriptionPlasmid for single guide RNA IVT
    Vector Typeplasmid
    Annotated Mapaddgene-plasmid-153997-sequence-304516
    Detection MethodNo. of sites detected
    Change-seq 1136
    Guide-seq 5
    Specificity Ratio0.153



    Publication Title
    CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

    This Guide: LAG3_site_03 is being used ...
    Project Initiative Contact PI
    Biological Effects Shengdar Q Tsai, PhD
    (St. Jude Children's Research Hospital)
    NIH Report