LAG3_site_09 | SCGE Toolkit

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SCGE ID	10000000162
Lab ID	LAG3_site_09
Source	lab IVT
Target Locus	LAG3
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GAAGGCTGAGATCCTGGAGG
Target Sequence + PAM	GAAGGCTGAGATCCTGGAGGNGG
Position	hg38/chr12:6773276-6773299

Spacer Sequence	GAAGGCUGAGAUCCUGGAGG
Spacer Length	20
Modifications	none

Forward Primer	CCCCCTCCCCTTGGCCTCTCTTTT
Reverse Primer	CTCAGGGCAGGGGACAGAGGACC

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	6849
Guide-seq	120

Specificity Ratio	0.001

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: LAG3_site_09 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)