SCGE ID10000000173
Lab IDPDCD1_site_10
Sourcelab IVT
Target LocusPDCD1
Specieshuman
DescriptionTargets endogenous human locus
Guide CompatibilitySpyCas9

Target SequenceGGAGAAGGTGGGGGGGTTCC
Target Sequence + PAMGGAGAAGGTGGGGGGGTTCCNGG
Positionhg38/chr2:241852942-241852965

Spacer SequenceGGAGAAGGUGGGGGGGUUCC
Spacer Length20
Modificationsnone

Forward PrimerCGGCCAGCTTGTCCGTCTGGTTG
Reverse PrimerCTTGCTGCCCGAGGGATGTGAGC


  • SpCas9


  • Ivt Construct SourceAddgene
    Vector Id153997
    Vector NamepCRL01
    Vector DescriptionPlasmid for single guide RNA IVT
    Vector Typeplasmid
    Annotated Mapaddgene-plasmid-153997-sequence-304516
    Detection MethodNo. of sites detected
    Change-seq 6838
    Guide-seq 61
    Specificity Ratio0.006



    Publication Title
    CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

    This Guide: PDCD1_site_10 is being used ...
    Project Initiative Contact PI
    Biological Effects Shengdar Q Tsai, PhD
    (St. Jude Children's Research Hospital)
    NIH Report