SCGE ID10000000186
Lab IDPTPN6_site_04
Sourcelab IVT
Target LocusPTPN6
Specieshuman
DescriptionTargets endogenous human locus
Guide CompatibilitySpyCas9

Target SequenceGGTTTCACCGAGACCTCAGT
Target Sequence + PAMGGTTTCACCGAGACCTCAGTNGG
Positionhg38/chr12:6951610-6951633

Spacer SequenceGGUUUCACCGAGACCUCAGU
Spacer Length20
Modificationsnone

Forward PrimerTCTGCCTGCCCAGACTAGCTGCA
Reverse PrimerCACCCTGGTTCTTGCGACTGGGC


  • SpCas9


  • Ivt Construct SourceAddgene
    Vector Id153997
    Vector NamepCRL01
    Vector DescriptionPlasmid for single guide RNA IVT
    Vector Typeplasmid
    Annotated Mapaddgene-plasmid-153997-sequence-304516
    Detection MethodNo. of sites detected
    Change-seq 333
    Guide-seq 1
    Specificity Ratio0.14



    Publication Title
    CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

    This Guide: PTPN6_site_04 is being used ...
    Project Initiative Contact PI
    Biological Effects Shengdar Q Tsai, PhD
    (St. Jude Children's Research Hospital)
    NIH Report