PTPN6_site_05 | SCGE Toolkit

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SCGE ID	10000000187
Lab ID	PTPN6_site_05
Source	lab IVT
Target Locus	PTPN6
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GAGACCCTGCTCAAGGGCCG
Target Sequence + PAM	GAGACCCTGCTCAAGGGCCGNGG
Position	hg38/chr12:6951642-6951665

Spacer Sequence	GAGACCCUGCUCAAGGGCCG
Spacer Length	20
Modifications	none

Forward Primer	ATTCCCTGCGCCCCCTTCCTCTC
Reverse Primer	GGCCCACCTACCTGACGGAGAGC

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	403
Guide-seq	3

Specificity Ratio	0.173

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: PTPN6_site_05 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)