Development System
TRAC_site_03 | SCGE Toolkit
Targets endogenous human locus
| 10000000194 |
| TRAC_site_03 |
| lab IVT |
| TRAC |
| human |
| Targets endogenous human locus |
| SpyCas9 |
|
| GAGAATCAAAATCGGTGAAT |
| GAGAATCAAAATCGGTGAATNGG |
| hg38/chr14:22547572-22547595 |
|
| GAGAAUCAAAAUCGGUGAAU |
| 20 |
| none |
|
| GCCTGGGTTGGGGCAAAGAGGGA |
| GTTGCTCCAGGCCACAGCACTGT |
| Ivt Construct Source | Addgene |
| Vector Id | 153997 |
| Vector Name | pCRL01 |
| Vector Description | Plasmid for single guide RNA IVT |
| Vector Type | plasmid |
| Annotated Map | addgene-plasmid-153997-sequence-304516 |
|
|
| Detection Method | No. of sites detected |
|---|
| Change-seq |
118
|
| Guide-seq |
2
|
|
|
| Publication Title |
|
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI |
This Guide: TRAC_site_03 is being used ...
| Project |
Initiative |
Contact PI |
|
|
|
Biological Effects |
Shengdar Q Tsai, PhD
(St. Jude Children's Research Hospital) |
|