SCGE ID10000000195
Lab IDTRAC_site_04
Sourcelab IVT
Target LocusTRAC
Specieshuman
DescriptionTargets endogenous human locus
Guide CompatibilitySpyCas9

Target SequenceGACACCTTCTTCCCCAGCCC
Target Sequence + PAMGACACCTTCTTCCCCAGCCCNGG
Positionhg38/chr14:22547756-22547779

Spacer SequenceGACACCUUCUUCCCCAGCCC
Spacer Length20
Modificationsnone

Forward PrimerACAGTGCTGTGGCCTGGAGCAAC
Reverse PrimerCCAGAACAAGGCTCACTGTTTCTTAGT


  • SpCas9


  • Ivt Construct SourceAddgene
    Vector Id153997
    Vector NamepCRL01
    Vector DescriptionPlasmid for single guide RNA IVT
    Vector Typeplasmid
    Annotated Mapaddgene-plasmid-153997-sequence-304516
    Detection MethodNo. of sites detected
    Change-seq 1444
    Guide-seq 77
    Specificity Ratio0.005



    Publication Title
    CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

    This Guide: TRAC_site_04 is being used ...
    Project Initiative Contact PI
    Biological Effects Shengdar Q Tsai, PhD
    (St. Jude Children's Research Hospital)
    NIH Report