TRAC_site_04 | SCGE Toolkit

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SCGE ID	10000000195
Lab ID	TRAC_site_04
Source	lab IVT
Target Locus	TRAC
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GACACCTTCTTCCCCAGCCC
Target Sequence + PAM	GACACCTTCTTCCCCAGCCCNGG
Position	hg38/chr14:22547756-22547779

Spacer Sequence	GACACCUUCUUCCCCAGCCC
Spacer Length	20
Modifications	none

Forward Primer	ACAGTGCTGTGGCCTGGAGCAAC
Reverse Primer	CCAGAACAAGGCTCACTGTTTCTTAGT

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	1444
Guide-seq	77

Specificity Ratio	0.005

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: TRAC_site_04 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)