TRAC_site_05 | SCGE Toolkit

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SCGE ID	10000000196
Lab ID	TRAC_site_05
Source	lab IVT
Target Locus	TRAC
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GTCGAGAAAAGCTTTGAAAC
Target Sequence + PAM	GTCGAGAAAAGCTTTGAAACNGG
Position	hg38/chr14:22549660-22549683

Spacer Sequence	GUCGAGAAAAGCUUUGAAAC
Spacer Length	20
Modifications	none

Forward Primer	AGGTCTGGGGTGGGAGAGTCAGC
Reverse Primer	AGGAGAGACCCCACCCAGCTCTG

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	139

Specificity Ratio	0.218

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: TRAC_site_05 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects Initiative	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)