SCGE ID10000000197
Lab IDTRBC1_site_01
Sourcelab IVT
Target LocusTRBC1
Specieshuman
DescriptionTargets endogenous human locus
Guide CompatibilitySpyCas9

Target SequenceGAACAAGGTGTTCCCACCCG
Target Sequence + PAMGAACAAGGTGTTCCCACCCGNGG
Positionhg38/chr7:142791700-142791723

Spacer SequenceGAACAAGGUGUUCCCACCCG
Spacer Length20
Modificationsnone

Forward PrimerCCCATTCCCAGGGCTCTCTCTCA
Reverse PrimerGTGCTGACCCCACTGTGCACCTC


  • SpCas9


  • Ivt Construct SourceAddgene
    Vector Id153997
    Vector NamepCRL01
    Vector DescriptionPlasmid for single guide RNA IVT
    Vector Typeplasmid
    Annotated Mapaddgene-plasmid-153997-sequence-304516
    Detection MethodNo. of sites detected
    Change-seq 865
    Specificity Ratio0.004



    Publication Title
    CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

    This Guide: TRBC1_site_01 is being used ...
    Project Initiative Contact PI
    Biological Effects Shengdar Q Tsai, PhD
    (St. Jude Children's Research Hospital)
    NIH Report