TRBC1_site_02 | SCGE Toolkit

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SCGE ID	10000000198
Lab ID	TRBC1_site_02
Source	lab IVT
Target Locus	TRBC1
Species	human
Description	Targets endogenous human locus
Guide Compatibility	SpyCas9

Target Sequence	GGTGCACAGTGGGGTCAGCA
Target Sequence + PAM	GGTGCACAGTGGGGTCAGCANGG
Position	hg38/chr7:142791845-142791867

Spacer Sequence	GGUGCACAGUGGGGUCAGCA
Spacer Length	20
Modifications	none

Forward Primer	CCCGAGGTCGCTGTGTTTGAGCC
Reverse Primer	CGGAAGTGGTTGCGGGGGTTCTG

Ivt Construct Source	Addgene
Vector Id	153997
Vector Name	pCRL01
Vector Description	Plasmid for single guide RNA IVT
Vector Type	plasmid
Annotated Map	addgene-plasmid-153997-sequence-304516

Detection Method	No. of sites detected
Change-seq	871

Specificity Ratio	0.647

Publication Title
CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity. NCBI

This Guide: TRBC1_site_02 is being used ...

Project	Initiative	Contact PI
A Novel Human T-Cell Platform to Define Biological Effects of Genome Editing Experiments A novel human T cell platform to define biological adverse effects of genome editing	Biological Effects	Shengdar Q Tsai, PhD (St. Jude Children's Research Hospital)