Summary

SCGE ID10000000206
Lab IDSTS118 (TLR-MCV1a; Sp_t2:Sp_c20_TLR_MCV1a)
Sourcein house production
Target LocusTLR MCV reporter
Speciestransgene
DescriptionTargets traffic light reporter transgene
Guide CompatibilitySpyCas9
Target SequenceGAGACAAATCACCTGCCTCG
Target Sequence + PAMGAGACAAATCACCTGCCTCGTGG
Full Guide Sequence

Sp_t2: AGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUU

Sp_c20_TLR_MCV1a: GAGACAAAUCACCUGCCUCGGUUUUAGAGCUAUGCU

Spacer SequenceGAGACAAAUCACCUGCCUCG
Spacer Length20
Modifications

Sp_t2: (mA)#(mG)#(mC)#(mA)(mU)(mA)(mG)(mC)(mA)(mA)(mG)(rU)(rU)(mA)(rA)(mA)(mA)(rU)(mA)(mA)(mG)(mG)(rC)(rU)(mA)(rG)(rU)(rC)(mC)(rG)(rU)(rU)(mA)(mU)(mC)(mA)(mA)(mC)(mU)(mU)(mG)(mA)(mA)(mA)(mA)(mA)(mG)(mU)(mG)(mG)(mC)(mA)(mC)(mC)(mG)(mA)(mG)(mU)(mC)(mG)(mG)(mU)(mG)(mC)#(mU)#(mU)#(mU)

Sp_c20_TLR_MCV1a: (mG)#(mA)#(mG)#(mA)(mC)(mA)(mA)(mA)(mU)(mC)(fA)(fC)(fC)(fU)(rG)#(rC)#(fC)(fU)(rC)#(mG)(mG)(rU)#(rU)#(rU)#(fU)(fA)(mG)(mA)(mG)(mC)(mU)(mA)(mU)#(mG)#(mC)#(mU)

Repeat SequenceGUUUUAGAGCUAUGCU
Anit-Repeat SequenceAGCAUAGCAAGUUAAAAU
Stemloop 1 SequenceAAGGCUAGUCCGU
Stemloop 2 SequenceAACUUGAAAAAGUG
Stemloop 3 SequenceGCACCGAGUCGGUGC

Other guides that target The same sequence

  • STS118 (TLR-MCV1a; Sp_t2-PS:Sp_c20_TLR_MCV1a)
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    Related Editors

  • SpyCas9-3xNLS

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    Projects & Experiments

    This Guide: STS118 (TLR-MCV1a; Sp_t2:Sp_c20_TLR_MCV1a) is being used ...
    Project Initiative Contact PI
    Delivery Systems Erik J Sontheimer, PhD
    (University of Massachusetts Medical School, Worcester)    		 NIH Report