Guide: STS118 (TLR-MCV1a; Sp_t2-PS:Sp_c20_TLR_MCV1a)
Summary
| SCGE ID | 10000000237 |
| Lab ID | STS118 (TLR-MCV1a; Sp_t2-PS:Sp_c20_TLR_MCV1a) |
| Source | in house production |
| Target Locus | TLR MCV reporter |
| Species | transgene |
| Description | Targets traffic light reporter transgene, CY3 labeled |
| Guide Compatibility | SpyCas9 |
| Target Sequence | GAGACAAATCACCTGCCTCG |
| Target Sequence + PAM | GAGACAAATCACCTGCCTCGTGG |
| Full Guide Sequence | Sp_t2-PS: AAUCGAAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUAUGCA Sp_c20_TLR_MCV1a: GAGACAAAUCACCUGCCUCGGUUUUAGAGCUAUGCU-Cy3; |
| Spacer Sequence | GAGACAAAUCACCUGCCUCG |
| Spacer Length | 20 |
| Modifications | Sp_t2-PS: (mA)#(mA)#(mU)#(mC)#(mG)#(mA)#(mA)#(mG)#(mC)#(mA)(mU)(mA)(mG)(mC)(mA)(mA)(mG)(rU)(rU)(mA)(rA)(mA)(mA)(rU)(mA)(mA)(mG)(mG)(rC)(rU)(mA)(rG)(rU)(rC)(mC)(rG)(rU)(rU)(mA)(mU)(mC)(mA)(mA)(mC)(mU)(mU)(mG)(mA)(mA)(mA)(mA)(mA)(mG)(mU)(mG)(mG)(mC)(mA)(mC)(mC)(mG)(mA)(mG)(mU)(mC)(mG)(mG)(mU)(mG)(mC)#(mU)#(mU)#(mU)#(mU)#(mA)#(mU)#(mG)#(mC)#(mA) Sp_c20_MCV1a: (mG)#(mA)#(mG)#(mA)(mC)(mA)(mA)(mA)(mU)(mC)(fA)(fC)(fC)(fU)(rG)#(rC)#(fC)(fU)(rC)#(mG)(mG)(rU)#(rU)#(rU)#(fU)(fA)(mG)(mA)(mG)(mC)(mU)(mA)(mU)#(mG)#(mC)#(mU)-Cy3 |
| Repeat Sequence | GUUUUAGAGCUAUGCU |
| Anit-Repeat Sequence | AAUCGAAGCAUAGCAAGUUAAAAU |
| Stemloop 1 Sequence | AAGGCUAGUCCGU |
| Stemloop 2 Sequence | AACUUGAAAAAGUG |
| Stemloop 3 Sequence | GCACCGAGUCGGUGCUUUUAUGCA |
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Projects & Experiments
This Guide: STS118 (TLR-MCV1a; Sp_t2-PS:Sp_c20_TLR_MCV1a) is being used ...| Project | Initiative | Contact PI | |
|---|---|---|---|
| Delivery Systems |
Erik J Sontheimer, PhD
(University of Massachusetts Medical School, Worcester) |
|