SCGE ID10000000216
Lab IDSaLoxP1-modified
SourceVector encoded
Target LocusAi9/Ai14/mTmG and related reporter transgene (within loxP)
Speciestransgene
DescriptionThis gRNA targets the mTmG, Ai9 and related transgenes at two sites
Guide CompatibilitySauCas9

Target SequenceACTTCGTATAATGTATGCTAT
Target Sequence + PAMACTTCGTATAATGTATGCTATACGAAG

Full Guide SequenceACUUCGUAUAAUGUAUGCUAUGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA
Spacer SequenceACUUCGUAUAAUGUAUGCUAU
Spacer Length21
ModificationsGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA gRNA scaffold sequence modification

  • SaLoxP1-unmodified

  • SaCas9



  • This Guide: SaLoxP1-modified is being used ...
    Project Initiative Contact PI
    Delivery Systems Benjamin E Deverman, PhD
    (Broad Institute)
    NIH Report