Guide: SaLoxP1-modified
Summary
SCGE ID | 10000000216 |
Lab ID | SaLoxP1-modified |
Source | Vector encoded |
Target Locus | Ai9/Ai14/mTmG and related reporter transgene (within loxP) |
Species | transgene |
Description | This gRNA targets the mTmG, Ai9 and related transgenes at two sites |
Guide Compatibility | SauCas9 |
Target Sequence | ACTTCGTATAATGTATGCTAT |
Target Sequence + PAM | ACTTCGTATAATGTATGCTATACGAAG |
Full Guide Sequence | ACUUCGUAUAAUGUAUGCUAUGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA |
Spacer Sequence | ACUUCGUAUAAUGUAUGCUAU |
Spacer Length | 21 |
Modifications | GUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA gRNA scaffold sequence modification |
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