SCGE ID10000000218
Lab IDSaLoxP2-modified
SourceVector encoded
Target LocusAi9/Ai14/mTmG and related reporter transgene (within loxP)
Speciestransgene
DescriptionThis gRNA targets the mTmG, Ai9 and related transgenes at two sites
Guide CompatibilitySauCas9

Target SequenceACTTCGTATAGCATACATTAT
Target Sequence + PAMACTTCGTATAGCATACATTATACGAAG

Full Guide SequenceACUUCGUAUAGCAUACAUUAUGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA
Spacer SequenceACUUCGUAUAGCAUACAUUAU
Spacer Length21
ModificationsGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA gRNA scaffold sequence modification

  • SaLoxP2-unmodified

  • SaCas9



  • This Guide: SaLoxP2-modified is being used ...
    Project Initiative Contact PI
    Delivery Systems Benjamin E Deverman, PhD
    (Broad Institute)
    NIH Report