SCGE ID10000000222
Lab IDR1-modified
SourceVector encoded
Target LocusAi9/Ai14 reporter transgene
Speciestransgene
DescriptionThis gRNA targets the Ai9 and related transgenes
Guide CompatibilitySauCas9

Target SequenceTACGAAGTTATATTAAGGGTT
Target Sequence + PAMTACGAAGTTATATTAAGGGTTCCGGAT

Full Guide SequenceUACGAAGUUAUAUUAAGGGUUGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA
Spacer SequenceUACGAAGUUAUAUUAAGGGUU
Spacer Length21
ModificationsGUUUAAGUACUCUGUGCUGGAAACAGCACAGAAUCUACUUAAACAAGGCAAAAUGCCGUGUUUAUCUCGUCAACUUGUUGGCGAGA gRNA scaffold sequence modification

  • R1-unmodified

  • SaCas9



  • This Guide: R1-modified is being used ...
    Project Initiative Contact PI
    Delivery Systems Benjamin E Deverman, PhD
    (Broad Institute)
    NIH Report