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DMD low gRNA-1 | SCGE Toolkit
One of two low-off target gRNAs delivred by dual gRNA AAV vector pAAV[2gRNA]-mCherry-U6>{SagRNA#1*}:{SagRNA#2}
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Guide: DMD low gRNA-1
Summary
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Summary
SCGE ID
10000000340
Lab ID
DMD low gRNA-1
Source
VectorBuilder
Target Locus
DMD
Species
human
Description
One of two low-off target gRNAs delivred by dual gRNA AAV vector pAAV[2gRNA]-mCherry-U6>{SagRNA#1*}:{SagRNA#2}
Guide Compatibility
SauCas9
Target Sequence
GTTAGCTCATATATTGACAGG
Target Sequence + PAM
GTTAGCTCATATATTGACAGGTGAAT
Position
hg38/X:32212049-32212069
Spacer Sequence
GUUAGCUCAUAUAUUGACAGG
Spacer Length
21
Modifications
none
Sequence Viewer
Related Editors
SaCas9 (AAV-SaCas9)
Projects & Experiments
This Guide: DMD low gRNA-1 is being used ...
Project
Initiative
Contact PI
Vascularized kidney organoids on chip for efficacy and toxicity testing of somatic genome editing
Experiments
Delivery of saCas9 and gRNAs to nephron epithelia by AAV2 in kidney organoids.
Biological Effects
Ryuji Morizane, MD, PhD
(Massachusetts General Hospital)