DMD low gRNA-1 | SCGE Toolkit

SCGE ID	10000000340
Lab ID	DMD low gRNA-1
Source	VectorBuilder
Target Locus	DMD
Species	human
Description	One of two low-off target gRNAs delivred by dual gRNA AAV vector pAAV[2gRNA]-mCherry-U6>{SagRNA#1*}:{SagRNA#2}
Guide Compatibility	SauCas9

Target Sequence	GTTAGCTCATATATTGACAGG
Target Sequence + PAM	GTTAGCTCATATATTGACAGGTGAAT
Position	hg38/X:32212049-32212069

Spacer Sequence	GUUAGCUCAUAUAUUGACAGG
Spacer Length	21
Modifications	none

This Guide: DMD low gRNA-1 is being used ...

Project	Initiative	Contact PI
Vascularized kidney organoids on chip for efficacy and toxicity testing of somatic genome editing Experiments Delivery of saCas9 and gRNAs to nephron epithelia by AAV2 in kidney organoids.	Biological Effects Initiative	Ryuji Morizane, MD, PhD (Massachusetts General Hospital)

Guide: DMD low gRNA-1