Model System: HEK-293T with Ai9 transient reporter assay
Summary
| SCGE ID | 13000000018 |
| Name | HEK-293T with Ai9 transient reporter assay |
| Species | Human |
| Type | Cell |
| Subtype | Immortalized |
| Description | HEK-293T cells transfected with an Ai9 inducible transgene reporter plasmid used to test gene editing activity by fluorescence. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. |
| Parental Origin | HEK-293T |
| Transgene | Ai9 plasmid |
| Transgene Description | A mouse Rosa26 targeting vector plasmid with CAG-loxP-stop-loxP-tdtomato reporter cassette which is activated by Cre recombination or double strand breaks on both sides of the stop cassette and NHEJ repair |
| Reporter | tdTomato |
| Annotated Map | addgene-plasmid-22799-sequence-115994.gbk |
Projects & Experiments
This Model System: HEK-293T with Ai9 transient reporter assay is being used ...| Project | Initiative | Contact PI | |
|---|---|---|---|
| Delivery Systems Initiative |
Guang-Ping Gao, PhD
(University of Massachusetts Medical School, Worcester) |
|