Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
Filters ..
  Editors
 Cre recombinase
 SpCas9
Guide Target Locus
 Ai9/Ai14 reporter transgene
  Guides
 sgAi9L
 sgAi9R
Delivery Systems
 Lipofectamine 3000
Models
 HEK-293T with Ai9 transient reporter assay
Sex
 Female
  Vectors
 pAAV.pU1a-SpCas9
 pH509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1)

Results

Testing_AAV5_for_activation_of_tdTomato_in_HEK293T_cells
Editing Efficiency Delivery Efficiency
Record Id Condition Sex Editor Model Delivery Target Locus Guide Vector Dosage Editing Efficiency Signal Delivery Efficiency Signal Image
15000000409 Condition 1 Female HEK-293T with Ai9 transient reporter assay Lipofectamine 3000 Ai9/Ai14 reporter transgene sgAi9RsgAi9L pH509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) 2 micrograms of plasmid into 80,000 cells/well
15000000410 Condition 2 Female SpCas9 HEK-293T with Ai9 transient reporter assay Lipofectamine 3000 Ai9/Ai14 reporter transgene sgAi9RsgAi9L pAAV.pU1a-SpCas9; pH509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1) 2 micrograms of plasmid into 80,000 cells/well
15000000411 Condition 3 Female Cre recombinase HEK-293T with Ai9 transient reporter assay Lipofectamine 3000 2 micrograms of plasmid into 80,000 cells/well

Develop AAV dual sgRNA vector for Ai9 LSL-tdTomato reporter

Methods: 12well plate, 80,000cells per well. Lipo 3000. total 2ug plasmid per well (SpCas9 : sgRNA : Ai9 plasmid=1:1:1, equal mole). 72hours later take image.