Model System: HEK-293T-disrupted_GFP-mcherry-Puro
Summary
| SCGE ID | 13000000026 |
| Name | HEK-293T-disrupted_GFP-mcherry-Puro |
| Species | Human |
| Type | Cell |
| Subtype | Immortalized |
| Description | HEK293T cells with an integrated reporter for TLR1 reporter editing. HEK293T is an epithelial-like cell that was isolated from the kidney of a patient. |
| Parental Origin | HEK-293T (RRID:CVCL_0063) |
| Transgene | Traffic Light Reporter 1.1 (RRID:Addgene_31480) |
| Transgene Description | A Lenti reporter transgene was stably introduced into HEK-293T cells by random viral integration using puromycin selection. The editing of the reporter produces mcherry fluorescent protein after NHEJ repair pathway occurs. |
| Reporter | mCherry/GFP traffic light |
| Annotated Map | TLR1 plasmid |
Projects & Experiments
This Model System: HEK-293T-disrupted_GFP-mcherry-Puro is being used ...| Project | Initiative | Contact PI | |
|---|---|---|---|
| Delivery Systems Initiative |
Erik J Sontheimer, PhD
(University of Massachusetts Medical School, Worcester) |
|