HEK-293-TgEF1a-eGFP-BSD | SCGE Toolkit

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Summary Projects & Experiments

SCGE ID	13000000014
Name	HEK-293-TgEF1a-eGFP-BSD
Species	Human
Type	Cell
Subtype	Immortalized
Description	HEK293 cells with lentiviral insertion of EF1a promoter driving expression of eGFP and SV40 promoter driving expression of BSD. HEK293 is an epithelial-like cell that was isolated from the kidney of a patient.
Parental Origin	HEK293

Transgene	EF1a-eGFP-BSD
Transgene Description	EF1a promoter driving expression of eGFP and SV40 promoter driving expression of BSD

Publication Title
CRISPR-CasΦ from huge phages is a hypercompact genome editor. NCBI

This Model System: HEK-293-TgEF1a-eGFP-BSD is being used ...

Project	Initiative	Contact PI
Expanding CRISPR-Cas Editing Technology through Exploration of Novel Cas Proteins and DNA Repair Systems Experiments Testing newly discovered Biggie Phage editors in human cells	Genome Editors Initiative	Jennifer A Doudna, PhD Jillian Banfield, PhD (University of California, Berkeley)