Model System: Mouse Embryonic Fibroblasts
Summary
| SCGE ID | 13000000025 |
| Name | Mouse Embryonic Fibroblasts |
| Species | Mouse |
| Type | Cell |
| Subtype | Primary cells |
| Description | Primary cell line |
| Parental Origin | not specified |
| Source | In-house: https://jacks-lab.mit.edu/protocols/making_mefs |
| Transgene | mTmG: CAG-lox-tdTomato-lox-GFP |
| Transgene Description | mTmG is a double-fluorescent reporter that expresses membrane-targeted tdTomato prior to CRISPR-mediated deletion of the ORF. after genomic cleavage by Cas9 at 2 sites flanking tdTomato, the membrane-targeted GFP is expressed. The reporter is integrated into Rosa26 locus |
| Reporter | tdTomato/GFP double reporter |
| Annotated Map | mTmG plasmid |
Projects & Experiments
This Model System: Mouse Embryonic Fibroblasts is being used ...| Project | Initiative | Contact PI | |
|---|---|---|---|
| Delivery Systems |
Erik J Sontheimer, PhD
(University of Massachusetts Medical School, Worcester) |
|