Development System Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts | SCGE Toolkit Chemically modified crRNA and tracrRNA were delivered by electroporation to embryonic fibroblasts harvested from the mTmG reporter mouse. Gene editing was determined by reporter activation.
Experiment: Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts
PI:
Erik J Sontheimer, PhD
Chemically modified crRNA and tracrRNA were delivered by electroporation to embryonic fibroblasts harvested from the mTmG reporter mouse. Gene editing was determined by reporter activation.
Editing Assay:
- Calculated editing efficiency by flow cytometry analysis of GFP and RFP.
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Results
SCGE Toolkit downloaded on: 2023/03/22 12:09:15; Please cite the Somatic Cell Genome Editing Consortium Toolkit NIH HG010423 when using publicly accessible data in formal presentation or publication. SCGE Experment ID: 18000000018. PI:
Erik J Sontheimer PhD
