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Experiment: Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts

PI:   Erik J Sontheimer, PhD 
 
 

Chemically modified crRNA and tracrRNA were delivered by electroporation to embryonic fibroblasts harvested from the mTmG reporter mouse. Gene editing was determined by reporter activation.
Editing Assay: - Calculated editing efficiency by flow cytometry analysis of GFP and RFP.

Filter Results ..
Editors
 SpyCas9-3xNLS
Guide Target Locus
 mTmG reporter transgene
Guides
 STS159 (mTmG; Sp_t2:Sp_c20_mTmG)
Models
 Mouse Embryonic Fibroblasts
Sex
 Female

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Results

Testing_newly_chemically_modified_crRNA_and_tracrRNA_in_mTmG_mouse_embryonic_fibroblasts
Editing Efficiency
Record Id Condition Sex Editor Model Target Locus Guide Dosage Editing Efficiency % of GFP+ cells Image
15000000550 STS159 (mTmG) (2 pmol RNP) Female SpyCas9-3xNLS Mouse Embryonic Fibroblasts mTmG reporter transgene STS159 (mTmG; Sp_t2:Sp_c20_mTmG) 2 pmol RNP
15000000551 STS159 (mTmG) (20 pmol RNP) Female SpyCas9-3xNLS Mouse Embryonic Fibroblasts mTmG reporter transgene STS159 (mTmG; Sp_t2:Sp_c20_mTmG) 20 pmol RNP