Development System Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia | SCGE Toolkit In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.
Experiment: Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia
PI:
Paul B McCray, Jr, MD
In vivo shuttle peptide delivery of Cas9 and Cas12a RNPs in mouse airway epithelia. Gene editing was quantified by the GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.
Editing Assay:
- Quantitation of GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.