Experiment Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia. Experiment Condition S10, GFP, 2X Delivery Assay Description: Quantification of GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells. Tissue Measured: epithelium of bronchiole Measurement Type: Delivery Efficiency Record ID 15000000035 Editor GFP-NLS Delivery System S10 Delivery System Subtype Amphiphilic peptide Guide Vector Model Species Mouse Model Name BALB/c Application Method nasal instillation Application Site airway epithelium Dosage shuttle peptide (40 µM); GFP-NLS (20 µM) Injection Frequency twice Injection Rate over an 8 hour period Injection Volume 50 microliters Days post injection 18 hours Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description cilia (α-tubulin); F-actin (phalloidin stain); nuclei (DAPI) Measured Values Samples Size: 4   Replicate Result (Delivery Efficiency) Measurement Units: % of cells Mean 29.75  1 31.7  2 31.1  3 26.5  4 29.7