Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
Home / Project
 

Experiment: Testing Shuttle Peptides ability to deliver GFP-NLS to airway epithelia.

PI:   Paul B McCray, Jr, MD 

Description: Delivery of GFP via shuttle peptides to mouse airway epithelium via nasal instilation. Delivery efficiency was quantified in large and small airways by counting the number of GFP positive cells divided by the number of DAPI cells.
Delivery Assays: Quantification of GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells.
Download: Submitted files
Publications: 
  • Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI
Filters ..
  Tissues
Respiratory
  • Epithelium of bronchiole
  • Epithelium of main bronchus
Editors
 GFP-NLS
Delivery Systems
 S10
Models
 BALB/c mouse
Select experimental variable to highlight records on the chart: 
 

Note: Hover over the bars to view additional information

Results
Testing_Shuttle_Peptides_ability_to_deliver_GFP-NLS_to_airway_epithelia._
Delivery Efficiency
Record Id Condition Tissue Editor Model Delivery Dosage Injection Frequency Delivery Efficiency % of cells Image
15000000033 S10, GFP, 1X Delivery epithelium of bronchiole GFP-NLS BALB/c mouse S10 shuttle peptide (40 µM); GFP-NLS (20 µM) once
15000000035 S10, GFP, 2X Delivery epithelium of bronchiole GFP-NLS BALB/c mouse S10 shuttle peptide (40 µM); GFP-NLS (20 µM) twice
15000000280 S10, GFP, 1X Delivery epithelium of main bronchus GFP-NLS BALB/c mouse S10 shuttle peptide (40 µM); GFP-NLS (20 µM) once
15000000281 S10, GFP, 2X Delivery epithelium of main bronchus GFP-NLS BALB/c mouse S10 shuttle peptide (40 µM); GFP-NLS (20 µM) twice

Associated Publications
Publication Title
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI

GFP-NLS protein delivery to mouse airways using S10 peptide. a Fluorescence image of lung tissue section 18 h following two intranasal doses of [S10]: 40μM; [GFP]: 20μMin50μl; ×2 magnification. Insets show the large and small airways at ×20 magnification. b,c Localization of GFP in different cell types. GFP co-localized with specific markers of cilia (α-tubulin, red), F-actin (phalloidin stain, gray), and nuclei (DAPI, blue) in large (b) and small airway epithelia (c). Non-ciliated cells were identified by the absence of α-tubulin staining; ×40 magnification. Arrowheads indicate ciliated cells (α-tubulin); arrows indicate non-ciliated cells. n=4 mice per group. d GFP localization in distal lung region. Co-localization of GFP and SP-C (red), a marker of alveolar type II cells, F-actin (phalloidin stain, gray), and nuclei (DAPI, blue); ×40 magnification. White arrows indicate co-localization of GFP and SP-C. e Quantitation of GFP+ cells in large and small airways following 1 or 2 deliveries of GFP protein. Results are presented as mean ± SE; n=4 mice per group. Pubmed Link