Experiment: Testing virus region 4 (VR4) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice.
PI: Aravind Asokan, PhD
Description: C57BL/6 mice (N=3) were injected intravenously at a dose of 5e13 vg/kg per mouse with a self-complementary AAV9 or ccAAV vector encoding an mCherry reporter. The biodistribution of of virus transduction was chacterized in various tissues and cell types by fluorescence imaging quantification.
Delivery Assays:
- Native fluorescence was quantified using Image J. For each animal, 6 images were taken from 2 sections and used to calculate corrected total cell fluorescence using the formula: Integrated Density – (Area of selected image X Mean fluorescence of background readings).
- Relative neuronal transduction levels were quantified by counting the number of transduced neurons, identified based on morphology, for each brain region per 50um sagittal section
Parent Project: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing
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Other experiments in this project: 6
- Cre Recombinase dose escalation study in Ai9 mice
- Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)
- Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter)
- Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
- Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
- Testing virus region 8 (VR8) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice.
Download: Submitted files
Analyze Data Sets Available for this Experiment
Renal/Urinary
| Kidney |
| Liver |
| Heart |
| Skeletal muscle tissue |
| Cerebral cortex (neuron) |
| Cerebral cortex (astrocyte) |
| Cerebellum (neuron) |
| Cerebellum (astrocyte) |
| Hippocampal formation (neuron) |
| Hippocampal formation (astrocyte) |
| Dorsal plus ventral thalamus (neuron) |
| Dorsal plus ventral thalamus (astrocyte) |
| Striatum (neuron) |
| Striatum (astrocyte) |
| Midbrain (neuron) |
| Midbrain (astrocyte) |
Organ System Overview
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