Experiment Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells Experiment Condition S10 Assay Description: Calculated editing efficiency by % of NGS reads that showed an indel Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000941 Editor AsCas12a (Feldan Therapeutics) Delivery System S10 Delivery System Subtype Amphiphilic peptide Guide 3849 45-5' Vector Model Species Human Model Name Primary Airway Epithelia Application Method Application Site Dosage 2uM gRNA, 1.33uM Cas12a, 40uM Shuttle Injection Frequency Injection Rate Injection Volume Days post injection Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 27   Replicate Result (Editing Efficiency) Measurement Units: % of reads Mean 17.47  1 31.6  2 21  3 14.7  4 11.5  5 3.7  6 22.5  7 21.9  8 27.3  9 20.66  10 16.68  11 8.76  12 15.26  13 22.55  14 13.24  15 16.18  16 10.7  17 6.46  18 10.77  19 10.85  20 15.59  21 11.71  22 12.7100000381469  23 19.829999923706  24 25.1200008392333  25 29.63  26 22.13  27 28.69