Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells |
S10 |
Calculated editing efficiency by % of NGS reads that showed an indel |
|
Editing Efficiency |
15000000941 |
|
AsCas12a (Feldan Therapeutics) |
S10 |
Amphiphilic peptide |
3849 45-5' |
|
|
Human |
Primary Airway Epithelia |
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2uM gRNA, 1.33uM Cas12a, 40uM Shuttle |
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RNP |
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Measured Values |
Samples Size: | 27 |
|
Mean |
17.47  |
1 |
31.6  |
2 |
21  |
3 |
14.7  |
4 |
11.5  |
5 |
3.7  |
6 |
22.5  |
7 |
21.9  |
8 |
27.3  |
9 |
20.66  |
10 |
16.68  |
11 |
8.76  |
12 |
15.26  |
13 |
22.55  |
14 |
13.24  |
15 |
16.18  |
16 |
10.7  |
17 |
6.46  |
18 |
10.77  |
19 |
10.85  |
20 |
15.59  |
21 |
11.71  |
22 |
12.7100000381469  |
23 |
19.829999923706  |
24 |
25.1200008392333  |
25 |
29.63  |
26 |
22.13  |
27 |
28.69  |
| |
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