Experiment Testing AAV5 for activation of tdTomato in HEK293T cells Experiment Condition Condition 2 Assay Description: Three plasmids 1) AAV vector expressing dual sgRNAs and eGFP, 2) AAV vector expressing SpCas9 and 3) Ai9 transient reporter assay plasmid were transiently transfected into HEK293T cells. Images were taken for tdTomato expression after 72 hours Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000410 Editor SpCas9 Delivery System Lipofectamine 3000 Delivery System Subtype Commercial Reagent Guide sgAi9R; sgAi9L Vector pAAV.pU1a-SpCas9; pH509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1); Model Species Human Model Name HEK-293T with Ai9 transient reporter assay Application Method Lipofectamine 3000 Application Site Dosage 2 micrograms of plasmid into 80,000 cells/well Injection Frequency Injection Rate Injection Volume Days post injection 72 hours Editor Format plasmids Antidote Id Antidote Description Measured Values Samples Size: 1   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 1 of 1 Present  1 Present  Mean 1 of 1 Present  1 Present