Experiment Testing AAV5 for activation of tdTomato in mouse airway Experiment Condition Dual AAV Assay Description: Two AAVs encoding sgRNAs and SpCas9 were introduced by intratracheal injection into Ai9 mice. After 4 weeks, lung tissue was harvested and prepared by sectioning and immunohistochemical staining for tdTomato. Editing efficiency calculated by counting fluorescent cells (RFP) compared to DAPI stained cells. Tissue Measured: epithelium of main bronchus Cell Type : epithelial cell of lung Measurement Type: Editing Efficiency Record ID 15000001636 Editor SpCas9 Delivery System Delivery System Subtype Guide sgAi9L; sgAi9R Vector AAV.pU1a-SpCas9; H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1); Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 8 weeks Sample Sex Female Application Method inhalation Application Site airway epithelium Dosage 6e10 gc/each AAV per mouse by intratracheal injection Injection Frequency once Injection Rate n/a Injection Volume 40 microliters Days post injection 4 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Antibody RRID Antibody Other ID Cell Signaling Technology Cat# 2956 Antibody Description GFP (D5.1) XP Rabbit mAb antibody Antibody RRID Antibody Other ID Rockland Cat# 600-401-379 Antibody Description Anti-RFP (RABBIT) Antibody Measured Values Samples Size: 5   Replicate Result (Editing Efficiency) Measurement Units: % cells Mean 24.2  1 20.5  2 21.5  3 25.4  4 23.1  5 30.5