Testing AAV5 for activation of tdTomato in mouse airway |
Dual AAV |
Two AAVs encoding sgRNAs and SpCas9 were introduced by intratracheal injection into Ai9 mice. After 4 weeks, lung tissue was harvested and prepared by sectioning and immunohistochemical staining for GFP. Delivery efficency was calculated by counting GFP+ cells compared to DAPI stained cells. |
epithelium of main bronchus |
epithelial cell of lung |
Delivery Efficiency |
15000001637 |
|
SpCas9 |
|
|
sgAi9R; sgAi9L |
AAV.pU1a-SpCas9; H509 AAVsc-u6-sgAI9L-U6-AI9R-U1A-EGFP (1); |
|
Mouse |
B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J |
|
inhalation |
airway epithelium |
6e10 gc/each AAV per mouse by intratracheal injection |
once |
n/a |
40 microliters |
4 weeks |
AAV vector encoded |
|
|
|
|
Cell Signaling Technology Cat# 2956 |
GFP (D5.1) XP Rabbit mAb antibody |
|
|
Rockland Cat# 600-401-379 |
Anti-RFP (RABBIT) Antibody |
|
Measured Values |
Samples Size: | 5 |
|
Mean |
31.66  |
1 |
30.2  |
2 |
36.3  |
3 |
29.1  |
4 |
29.5  |
5 |
33.2  |