Experiment Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. Experiment Condition CM18-PTD4, Cas12a, CFTR Assay Description: Calculated editing efficiency mean of 3 biological replicates by sanger sequencing. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000538 Editor AsCas12a (Feldan Therapeutics) Delivery System CM18-PTD4 Delivery System Subtype Amphiphilic peptide Guide g-45_C12a Vector Model Species Human Model Name Primary Airway Epithelia Application Method In Media Application Site Dosage 1.33uM Cas12a/2.0uM gRNA/40uM Shuttle Peptide Injection Frequency Injection Rate Injection Volume Days post injection 72 hours Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 1   Replicate Result (Editing Efficiency) Measurement Units: % indel Mean 9.00  1 9.0