Development System
S85, Cas9, CFTR | SCGE Toolkit
Database of study and experiment data generated by the Somatic Cell Genome Editing Consortium
| Gene editing in vitro by various peptide variants delivering Cas RNPs to primary Human airway epithelia cells. |
| S85, Cas9, CFTR |
| Calculated editing efficiency mean of 3 biological replicates by sanger sequencing. |
|
| Editing Efficiency |
| 15000000545 |
|
| SpCas9 (Feldan Therapeutics) |
| S85 |
| Amphiphilic peptide |
| g-11_C9 |
|
|
| Human |
| Primary Airway Epithelia |
|
| In Media |
|
| [RNP]: 2.5 µM; [gRNA]: 2.0 μM; [Peptide]: 40uM |
|
|
|
| 72 hours |
| ribonucleoprotein (RNP) |
|
|
|
| Measured Values |
| Samples Size: | 1 |
| |
| Mean |
9.00  |
| 1 |
9.0  |
| |
|
| Publication Title |
|
Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia. NCBI |