Experiment Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts Experiment Condition STS159 (mTmG) (2 pmol RNP) Assay Description: Calculated editing efficiency by flow cytometry analysis of GFP and RFP. Tissue Measured: Measurement Type: Editing Efficiency Record ID 15000000550 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS159 (mTmG; Sp_t2:Sp_c20_mTmG) Vector Model Species Mouse Model Name MEF Application Method electroporation Application Site Dosage 2 pmol RNP Injection Frequency Injection Rate Injection Volume Days post injection 48-72 hours Editor Format protein, RNA Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: % of GFP+ cells Mean 24.2  1 24.8  2 23.6