Experiment: Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain
PI: Erik J Sontheimer, PhD
Description: Chemically modified crRNA and tracrRNA were injected into the striatum of TLR-MCV mice and the distribution of RNP was imaged.
Delivery Assays:
Delivery and uptake testing of C20-Cy3/T2-PS (red fluorescence) and SpyCas9 by immunohistochemical staining (green fluorescecnce) after intrastriatal injections
Parent Project: Enhancing CRISPR Gene Editing in Somatic Tissues by Chemical Modification of Guides and Donors
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Other experiments in this project: 10
- Testing newly chemically modified crRNA and tracrRNA in mTmG mouse embryonic fibroblasts
- Testing newly chemically modified crRNA and tracrRNA in mouse Hepa 1-6 cells
- Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain
- Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
- Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
- Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
- Testing preparation for independent validation at The Jackson Laboratory Small Animal Testing Center
- Testing newly chemically modified crRNA and tracrRNA to activate the TLR reporter in human cells
- Testing newly chemically modified crRNA and tracrRNA to activate the TLR1 reporter in human cells
- Testing newly chemically modified crRNA and tracrRNA in mouse Neuro 2A cells
Download: Submitted files
Organ System Overview
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