Development System Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain | SCGE Toolkit Chemically modified crRNA and tracrRNA were injected into the striatum of TLR-MCV mice and the distribution of RNP was imaged.
Experiment: Delivery of RNP containing chemically modified crRNA C20 with chemically modified tracrRNA T2-PS to determine the RNP distribution in TLR-MCV mouse brain
PI:
Erik J Sontheimer, PhD
Chemically modified crRNA and tracrRNA were injected into the striatum of TLR-MCV mice and the distribution of RNP was imaged.
Delivery Assays:
- Delivery and uptake testing of C20-Cy3/T2-PS (red fluorescence) and SpyCas9 by immunohistochemical staining (green fluorescecnce) after intrastriatal injections