Experiment Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Experiment Condition cc47-SaCas9, Male Assay Description: Fluorescent imaging for tdTomato+ cells Tissue Measured: brown adipose tissue Measurement Type: Editing Efficiency Record ID 15000001249 Editor SauCas9 Delivery System Delivery System Subtype Guide Ai9 SaCas9 Guide A; Ai9 SaCas9 Guide B Vector AAVcc47-SaCas9-Ai9; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous, tail vein Application Site liver and heart Dosage 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) Injection Frequency once Injection Rate Injection Volume 200 microliters Days post injection 4 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 6   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 1 One red cell; 2 No red cells; 1 A few red cells; 2 Significant red cells  1 One red cell  2 No red cells  3 No red cells  4 A few red cells  5 Significant red cells  6 Significant red cells