Experiment Independent validation for Asokan Delivery Team: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing. Experiment Condition cc47-Cre Pos control, Male Assay Description: Editing efficiency calculated by counting fluorescent cells (RFP) compared to DAPI stained nuclei Tissue Measured: liver Cell Type : hepatocyte Measurement Type: Editing Efficiency Record ID 15000001292 Editor Cre recombinase Delivery System Delivery System Subtype Guide Vector AAVcc47-Cre; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Sample Age 8 weeks Sample Sex Male Application Method intravenous, tail vein Application Site liver and heart Dosage 1.0x10^14 vg copies/kg (vg/kg) (2.0x10^12/20g mouse) Injection Frequency once Injection Rate Injection Volume 200 microliters Days post injection 4 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 2 of 2 Significant red cells  1 Significant red cells  2 Significant red cells