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ExperimentCre Recombinase dose escalation study in Ai9 mice
Experiment ConditionAAVcc47-CMV-Cre, 1e11vg, Male
Assay Description: Delivery efficency was calculated by measuring fluorescent intensity in tdTomato+ cells from multiple images and sections in liver, heart, skeletal muscle
Tissue Measured:liver
Measurement Type:Delivery Efficiency
Record ID15000000980

EditorCre recombinase
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAVcc47-CMV-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Siteliver and heart
Dosage1e11vg
Injection Frequencyonce
Injection Rate
Injection Volume200 microliters
Days post injection4 weeks
Editor FormatAAV vector encoded
Antidote Id
Antidote Description

Measured Values
Samples Size: 3
 
Replicate Result (Delivery Efficiency)
Measurement Units: Corrected total cell fluorescence
Mean 4629745882.67 
1 6500827753 
2 5513267295 
3 1875142600 
  

Publication Title
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI