Experiment: Cre Recombinase dose escalation study in Ai9 mice
PI: Aravind Asokan, PhD
Description: A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 mice. We injected n=3 at three different doses (1e10, 1e11, 1e12 vg) and harvested organs 4 weeks post injection. Fluorescence intensity in liver, heart, and skeletal muscle was quantified with tiff based images in Image J and neuronal transduction from each vector was quantified at the 1e12vg dose by counting the number of tdTomato+ neurons and number of NeuN+ cells from multiple sections and images.
Delivery Assays:
- Delivery efficency was calculated by measuring fluorescent intensity in tdTomato+ cells from multiple images and sections in liver, heart, skeletal muscle
- Delivery efficency in brain was calculated by counting the total number of TdTomato+ neurons and total number of NeuN+ cells
Parent Project: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing
-
Other experiments in this project: 6
- Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CB promoter)
- Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter)
- Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 Cas9 to sgRNA ratio (CMV promoter) and self complementary sgRNA vector.
- Comparing CRISPR/Cas9 gene editing efficiencies between AAV9 and AAVcc47 in Ai9 mice with a 1:3 Cas9 to sgRNA ratio (CMV promoter)
- Testing virus region 8 (VR8) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice.
- Testing virus region 4 (VR4) mutant cross-species compatible Adeno Associated Viruses (ccAAVs) in mice.
Download: Submitted files
Analyze Data Sets Available for this Experiment
Liver and Biliary
| Liver (TARGET) |
| Heart (TARGET) |
| Skeletal muscle tissue |
| Cerebellum (neuron) |
| Hippocampal formation (neuron) |
| Cerebral cortex (neuron) |
Organ System Overview
|