Experiment: Cre Recombinase dose escalation study in Ai9 mice
PI: Aravind Asokan, PhD| A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 mice. We injected n=3 at three different doses (1e10, 1e11, 1e12 vg) and harvested organs 4 weeks post injection. Fluorescence intensity in liver, heart, and skeletal muscle was quantified with tiff based images in Image J and neuronal transduction from each vector was quantified at the 1e12vg dose by counting the number of tdTomato+ neurons and number of NeuN+ cells from multiple sections and images. |
| Delivery Assays: |
- Delivery efficency was calculated by measuring fluorescent intensity in tdTomato+ cells from multiple images and sections in liver, heart, skeletal muscle - Delivery efficency in brain was calculated by counting the total number of TdTomato+ neurons and total number of NeuN+ cells |
Organ System Overview
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