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ExperimentCre Recombinase dose escalation study in Ai9 mice
Experiment ConditionAAVcc47-CMV-Cre, 1e12vg, Female
Assay Description: Delivery efficency in brain was calculated by counting the total number of TdTomato+ neurons and total number of NeuN+ cells
Tissue Measured:cerebral cortex
Cell Type :neuron
Measurement Type:Delivery Efficiency
Record ID15000000991

EditorCre recombinase
Delivery SystemAAV
Delivery System SubtypeViral vector
Guide
VectorAAVcc47-CMV-Cre;

Model SpeciesMouse
Model NameB6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J

Application Methodintravenous
Application Siteliver and heart
Dosage1e12vg
Injection Frequencyonce
Injection Rate
Injection Volume200 microliters
Days post injection4 weeks
Editor FormatAAV vector encoded
Antidote Id
Antidote Description

Measured Values
Samples Size: 2
 
Replicate Result (Delivery Efficiency)
Measurement Units: % of cells
Mean 2.79 
1 2.829658343 
2 2.745178815 
  

Publication Title
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI
Cross-species evolution of a highly potent AAV variant for therapeutic gene transfer and genome editing. NCBI