Experiment Comparing CRISPR/Cas9 gene editing efficencies between AAV9 and AAVcc47 in Ai9 mice with a 1:1 cas9 to sgRNA ratio (CMV promoter) Experiment Condition AAV9, Female Assay Description: Editing efficiency calculated by counting fluorescent cells (TdTomato) compared to DAPI stained cells. Tissue Measured: heart Measurement Type: Editing Efficiency Record ID 15000001018 Editor SaCas9-Lagor Delivery System Delivery System Subtype Guide Vector AAV9-Ai9-sgRNA1 + sgRNA2; AAV9-CMV-SaCas9; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor ^ tm9(CAG-tdTomato)Hze/J Application Method intravenous Application Site liver and heart Dosage 1.5e12vg each vector mixed 1:1 Injection Frequency once Injection Rate Injection Volume 200 microliters Days post injection 4 weeks Editor Format AAV vector encoded Antidote Id Antidote Description Measured Values Samples Size: 1   Replicate Result (Editing Efficiency) Measurement Units: % of cells Mean 0.72  1 0.717481