Experiment Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Experiment Condition ssAAV5-sgB.saCas9, Ai9 - SauSpyCas9, male Assay Description: Fluorescent imaging for tdTomato+ cells Tissue Measured: epithelium of bronchiole Cell Type : respiratory epithelial cell Measurement Type: Editing Efficiency Record ID 15000001042 Editor SaCas9 Delivery System Delivery System Subtype Guide BCM_ssAAV5-Sa_sgB Vector ssAAV5-sgB.saCas9; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe Application Method intratracheal intubation Application Site lung airway epithelium Dosage 1.7E11 viral genomes Injection Frequency once Injection Rate Injection Volume 40 microliters Days post injection 4 weeks Editor Format virus in sterile saline Antidote Id Antidote Description Measured Values Samples Size: 7   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 6 < 10 red cells detected; 1 Sporadic red cells not concentrated in airway epithelia  1 < 10 red cells detected  2 < 10 red cells detected  3 < 10 red cells detected  4 < 10 red cells detected  5 < 10 red cells detected  6 < 10 red cells detected  7 Sporadic red cells not concentrated in airway epithelia