Experiment Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Experiment Condition ssAAV5-sgB.saCas9, Ai9 - SauSpyCas9, male Assay Description: PCR detection of AAV-delivered Cas9 and GFP by agarose gel electrophoresis Tissue Measured: lung Cell Type : respiratory epithelial cell Measurement Type: Delivery Efficiency Record ID 15000001044 Editor SaCas9 Delivery System Delivery System Subtype Guide BCM_ssAAV5-Sa_sgB Vector ssAAV5-sgB.saCas9; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe Sample Age 8 weeks Sample Sex Male Application Method intratracheal intubation Application Site lung airway epithelium Dosage 1.7E11 viral genomes Injection Frequency once Injection Rate Injection Volume 40 microliters Days post injection 4 weeks Editor Format virus in sterile saline Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Delivery Efficiency) Measurement Units: Signal Mean 3 Negative 1 Positive (SaCas9)  1 Negative  2 Negative  3 Negative  4 Positive (SaCas9)