Experiment Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Experiment Condition ssAAV5-spCas9 + ssAAV5-sgA+sgB-U1A.GFP, Ai9 - SauSpyCas9, female Assay Description: PCR detection of AAV-delivered Cas9 and GFP by agarose gel electrophoresis Tissue Measured: lung Measurement Type: Delivery Efficiency Record ID 15000001076 Editor SpCas9 Delivery System Delivery System Subtype Guide BCM_ssAAV5-Sp_sgA; BCM_ssAAV5-Sp_sgB Vector ssAAV5-sgA+sgB-U1A.GFP; ssAAV5-spCas9; Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe Application Method Intratracheal Intubation Application Site lung airway epithelium Dosage 1.7E11 viral genomes Injection Frequency once Injection Rate Injection Volume 40 microliters Days post injection 4 weeks Editor Format virus in sterile saline Antidote Id Antidote Description Measured Values Samples Size: 5   Replicate Result (Delivery Efficiency) Measurement Units: Signal Mean 2 Negative 3 Positive (SpCas9 and GFP)  1 Negative  2 Negative  3 Positive (SpCas9 and GFP)  4 Positive (SpCas9 and GFP)  5 Positive (SpCas9 and GFP)