Experiment Independent validation for Gao Delivery Team: Testing ssAAV5 delivered intratracheally for editing activity in lung epithelia in Ai9 mice Experiment Condition saline Neg control, female Assay Description: Fluorescent imaging for tdTomato+ cells Tissue Measured: epithelium of bronchiole Cell Type : respiratory epithelial cell Measurement Type: Editing Efficiency Record ID 15000001112 Editor Delivery System Delivery System Subtype Guide Vector Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor^em1(CAG-tdTomato)JaHe Sample Sex Female Application Method intratracheal intubation Application Site lung airway epithelium Dosage Injection Frequency once Injection Rate Injection Volume 40 microliters Days post injection 4 weeks Editor Format Antidote Id Antidote Description Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 3 of 3 no red cells  1 No red cells  2 No red cells  3 No red cells