Experiment Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Experiment Condition D10, RNP, Male Assay Description: Editing efficiency in small (<50000 um2) airways such as bronchioles and large (>50000 um2) airways such as the main bronchus, calculated by counting fluorescent cells (GFP) compared to DAPI stained cells. Tissue Measured: epithelium of bronchiole Cell Type : respiratory epithelial cell Measurement Type: Editing Efficiency Record ID 15000001140 Editor SpCas9 Delivery System D10 Delivery System Subtype Amphiphilic peptide Guide SpyCas9 g-loxP2_C9 Vector Model Species Mouse Model Name B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J Application Method intranasal Application Site airway epithelium Dosage 40 micromolar peptide, 2.5 micromolar SpCas9, 2 micromolar RNA Injection Frequency twice, one day apart Injection Rate Injection Volume 50 microliters Days post injection 1 week Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 6   Replicate Result (Editing Efficiency) Measurement Units: % of cells Mean 19.45  1 19.84  2 22.65  3 16.92  4 19.61  5 18.26  6 19.42