Experiment Independent validation for McCray Delivery Team: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides Experiment Condition RNP only, Male Assay Description: Editing efficiency in small (<50000 um2) airways such as bronchioles and large (>50000 um2) airways such as the main bronchus, calculated by counting fluorescent cells (GFP) compared to DAPI stained cells. Tissue Measured: epithelium of bronchiole Cell Type : respiratory epithelial cell Measurement Type: Editing Efficiency Record ID 15000001238 Editor SpCas9 Delivery System Delivery System Subtype Guide SpyCas9 g-loxP2_C9 Vector Model Species Mouse Model Name B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J Application Method intranasal Application Site airway epithelium Dosage 2.5 micromolar SpCas9, 2 micromolar RNA Injection Frequency twice, one day apart Injection Rate Injection Volume 55 microliters Days post injection 1 week Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: % of cells Mean 0.0  1 0  2 0